13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry



Oral Presentation 16

TITLE: In vitro comparison of antimicrobial effect of three intracanal irrigants and diode laser on canals infected with Enterococcus faecalis.

AUTHORS: Sohraabi-Araghi KH, Ashofte-Yazdi K, Iran-Parvar K.                      

Tehran University of Medical Sciences/International Campus, School of Dentistry.

SOURCE:  Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S16.


* doi:10.4317/medoral.17643515






Introduction: Bacteria are the primary etiology of pulpal and periradicular pathosis. In endodontically trea-ted teeth with persistent infections only one or a few bacterial species are present of which the most important is Enterococcus faecalis. The aim of this study was to compare antibacterial efficacy of canal disinfectants including 5.25% sodium hypochlorite, 2% chlorhexi-dine, MTAD ( a mixture of doxycycline, citric acid and a detergent (Tween 80)) and 830 nm diode laser.

Materials & Methods: The canals of 135 extracted single rooted human teeth where prepared using rotary instruments. The canals were contaminated with Enterococcus faecalis for 4 weeks and then were divided into 4 groups of 30 teeth each, a positive control group containing 10 teeth and a negative control group of 5 teeth. After using the disinfectants, samples obtained from canals by paper points and also shaving the canal walls were cultured. The Kruskal-Wallis test was used to analyze the results. 

Results: The results showed the bacterial reduction as below: 99.97±0.14 for sodium hypochlorite, 99.65±1.13 for chlorhexidine, 97.56±6.36 for laser and 96.91±5.60 for MTAD. The count of CFU obtained from dentin shavings was: 16/96±91/23 for sodium hypochlorite, 82/73±186/63 for chlorhexidine, 47/26±112/21 for laser and 341/34±1139/83 for MTAD.

Conclusion: According to the results, sodium hypochlorite was the most effective agent against Enterococcus faecalis (p<0.004), but laser was better than the others.