13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 28

TITLE: In vitro evaluation of laser fluorescence performance for detection of artificial caries-affected dentin in permanent molars.

AUTHORS: Matos AB, Garbui BU, Simionato MRL, Azevedo CS.

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S176.

* doi:10.4317/medoral.17643676

http://dx.doi.org/10.4317/medoral.17643676

Abstract

The aim of this study was to validate DIAGNOdent pen to detect and quantify demineralization of artificially induced caries-affected human dentin by an in vitro microbiological method. Artificial lesions were induced by a S. mutans microbiological culture and the samples (n=28) were divided into 3 groups according to the demineralization time: G1, 7 days; G2, 14 days; G3, 21 days. The surface of all specimens was protected by an acid-resistant nail varnish, except for a window where the caries lesion was induced by the biofilm in a batch-culture model supplemented with 5% sucrose. The measurements with DIAGNOdent were performed three times and the device was calibrated against a ceramic reference (standard calibration) according to the manufacture’s specifications, and, afterwards, the zero value of fluorescence was obtained from a sound part of the occlusal dentin previously protected. Data were subjected to ANOVA test at a 5% of significance. Statistical analysis revealed i nsignificant difference between G1, G2 and G3 (p=0,166). In conclusion, DIAGNOdent was able to detect difference between artificially indiced caries-affected dentin and sound dentin, however, there were no difference between the experimental groups.