13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 62

TITLE: Photodynamic therapy of root canals with polymicrobial infection ex vivo.

AUTHORS: Aliu XH, Gavazi B, Stubljar D, Alojz I.

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S210.

* doi:10.4317/medoral.17643710

http://dx.doi.org/10.4317/medoral.17643710

Abstract

Bacterial infection plays an important role in dental pathology therefore the main goal is the elimination of infection. Elimination of pathogens from root canals during endodontic treatment is difficult, so PDT is coming as an alternative treatment.

Seventy six extracted single-rooted teeth were collected and stored in saline solution. Teeth were gathered from Faculty of Dentistry in Pristina.Crowns were removed using diamond disc and the roots were shorte-ned to a length of 15 mm. The canals were enlarged and irrigated with 3% sodium hypochlorite, sterilized with absolute alcohol and rinsed with 17% EDTA.Root canals were infected with 5Mc Farland E. faecalis (ATCC 29212) and C. albicans (ATCC 60193) and incubated at 37 C for 7 days.After incubation was applied Photosensiter into root canal for 1 minute. Then was used Helbo laser in intervals of 1, 3 and 5 minutes for Enterococcus and Candida.After laser application root canals were irrigated with 2,4 ml PBS and 1mM EDTA. For detection of bacteria was applied flow cytometry using BD Cell Viability Kit.

Forty three canals were included in the results. Twenty teeth were inoculated with E. faecalis while twenty three with C. albicans. Others were used as controls.For both microorganisms we have shoved that laser treatment destroys them at all intervals. The best disinfection was observed at longer application with PDT.

Our results ex vivo suggest that PDT is more effective in 5 minutes application.