13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 71

TITLE: Investigation of photodynamic and Nd: YAG laser effect against endodonticmicroorganisms.

AUTHORS: Dogandzhiyska V, Gueorgieva T, Gergova R, Angelov I, Dimitrov S.

Medical University-Faculty of dental medicine-Sofia, Bulgaria

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S219.

* doi:10.4317/medoral.17643719

http://dx.doi.org/10.4317/medoral.17643719

Abstract

Introduction For the success of endodontic treatment is important to achieve complete elimination of microorganisms. In recent years a new method of root canal disinfection is introduced - photo-activated disinfection(PAD). Also, it has been proved in numerous studies that an emission of laser light in the root canal have a bactericidal effect .

Aim of the study The aim of this study was to evaluate the effects of Nd:YAG laser and PAD with Fotosan on viability of problematic gram-positive and gram-negative bacteria in biofilm infected root canals.

Materials and methods In this study we used 40 freshly extracted human teeth . The root canals are prepared using K3-files nickel-titanium instruments . The teeth were infected with bacteria and incubated 48 hours to form biofilms. The teeth were divided in two groups-20 teeth treated with PAD and 20 teeth treated with Nd:YAG laser. The forming of biofilm and PAD/laser effect were determined by the scanning electron microscopy and with counting CFU/ml.

Results The most strongly antibacterial activity was achieved with PAD with Fotosan than treatment with Nd:YAG laser. The activity against Enterococcus faecalis and Staphylococcus aureus is the most prominent.

Conclusion The PAD with Fotosan and Nd:YAG laser treatment show a powerful antimicrobial activity against the most frequent endodontic pathogens and may be used with success for treatment of the bacterial biofilms in the root canals.