13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

 

 

-  Poster 107

TITLE: Laser irradiation reduces lipid accumulation in parotid glands of streptozotocin-induced diabetic rats.

AUTHORS: Arana-Chavez VE, Nicolau J, Simões A.                      

Oral Biology Laboratory, Department Of Dental Materials, School Of Dentistry, University Of São Paulo

SOURCE:  Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S255.

 

* doi:10.4317/medoral.17643755

http://dx.doi.org/10.4317/medoral.17643755

 

 

 

Abstract

Accumulation of lipid droplets into secretory cells of rat salivary glands has been related to salivary hypofunction in diabetes. The aim of this study was to evaluate the effect of laser irradiation (LI) upon lipid accumulation in parotid glands (PG) of streptozotocin-induced diabetic rats. Twenty four female rats were divided into 6 groups: D0, D5, D20; and C0, C5, C20, respectively, diabetic (D) and control animals (C). Diabetes was induced by administration of streptozotocin and confirmed later by the glycaemia results. Twenty-nine days after the induction, the PG of groups D5 and C5; D20 and C20, were irradiated with 5 and 20 J/cm2 of laser diode (660nm/100mW) respectively. On the following day, the rats were euthanized, the parotid glands dissected, fixed in a glutaraldehyde-formaldehyde fixative, embedded in JB-4 or Spurr resin, and examined by light (LM) and transmission electron (TEM) microscopy. Diabetic rats without irradiation (D0) showed lipid droplets accumulate d into the secretory cells. Ultrastructurally, they were 1-3 µm in diameter, non-membrane-bounded, and appeared irregularly located into the cytoplasm, in close relation with the regular organelles as mitochondria, rough endoplasmic reticulum cisternae and even with the nuclear envelope. However, it was decreased after 5 and 20 J/cm2 of laser irradiation. Based on the results of this study, LI appears as an auxiliary therapy for the control of oral diabetic complications as salivary gland hypofunction.