Leite KLF, Martins ML, de Medeiros MMD, Bezerra NVF, Brito CSM, de Almeida LFD, Cavalcanti YW. Red propolis hydroalcoholic extract inhibits the formation of Candida albicans biofilms on denture surface. J Clin Exp Dent. 2020;12(7):e626-31.







1. Chin VK, Lee TY, Rusliza B, Chong PP. Dissecting Candida albicans Infection from the Perspective of C. albicans Virulence and Omics Approaches on Host-Pathogen Interaction: A Review. Int J Mol Sci. 2016;17:E1643.
PMid:27763544 PMCid:PMC5085676


2. Emami E, Taraf H, de Grandmont P, Gauthier G, de Koninck L, Lamarche C, et al. The association of denture stomatitis and partial removable dental prostheses: a systematic review. Int J Prosthodont. 2012;25:113-119.


3. de Souza RF, de Freitas Oliveira Paranhos H, Lovato da Silva CH, Abu-Naba'a L, Fedorowicz Z, Gurgan CA. Interventions for cleaning dentures in adults. Cochrane Database Syst Rev. 2009:CD007395.


4. Hilgert JB, Giordani JM, de Souza RF, Wendland EM, D'Avila OP, Hugo FN. Interventions for the Management of Denture Stomatitis: A Systematic Review and Meta-Analysis. J Am Geriatr Soc. 2016;64:2539-2545.


5. Zhang LW, Fu JY, Hua H, Yan ZM. Efficacy and safety of miconazole for oral candidiasis: a systematic review and meta-analysis. Oral Dis. 2016;22:185-195.


6. Skupien JA, Valentini F, Boscato N, Pereira-Cenci T. Prevention and treatment of Candida colonization on denture liners: a systematic review. J Prosthet Dent. 2013;110:356-362.


7. Jackson S, Coulthwaite L, Loewy Z, Scallan A, Verran J. Biofilm development by blastospores and hyphae of Candida albicans on abraded denture acrylic resin surfaces. J Prosthet Dent. 2014;112:988-993.


8. Faot F, Cavalcanti YW, Mendonça e Bertolini M, Pinto L de R, da Silva WJ, Cury AA. Efficacy of citric acid denture cleanser on the Candida albicans biofilm formed on poly(methyl methacrylate): effects on residual biofilm and recolonization process. BMC Oral Health. 2014;14:77.
PMid:24957210 PMCid:PMC4076759


9. Ragher M, Prabhu UM, Ittigi JP, Naik R, Mahesh CS, Pradeep MR. Efficacy of Denture Cleansers on Impact Strength of Heat polymerized Acrylic Resins. J Pharm Bioallied Sci. 2017;9:S241-S5.
PMid:29284972 PMCid:PMC5731022


10. Sen T, Samanta SK. Medicinal plants, human health and biodiversity: a broad review. Adv Biochem Eng Biotechnol. 2015;147:59-110.


11. Challinor VL, Bode HB. Bioactive natural products from novel microbial sources. Ann N Y Acad Sci. 2015;1354:82-97.


12. Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Curr Med Chem. 2015;22:132-149.


13. Inui S, Hatano A, Yoshino M, Hosoya T, Shimamura Y, Masuda S, et al. Identification of the phenolic compounds contributing to antibacterial activity in ethanol extracts of Brazilian red propolis. Nat Prod Res. 2014;28:1293-1296.


14. Freires IA, de Alencar SM, Rosalen PL. A pharmacological perspective on the use of Brazilian Red Propolis and its isolated compounds against human diseases. Eur J Med Chem. 2016;110:267-279.


15. Silva FRG, Matias TMS, Souza LIO, Matos-Rocha TJ, Fonseca SA, Mousinho KC, et al. Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas. Braz J Biol. 2018:S1519-69842018005029101.


16. das Neves MV, da Silva TM, Lima EO, da Cunha EV, Oliveira EJ. Isoflavone formononetin from red propolis acts as a fungicide against Candida sp. Braz J Microbiol. 2016;47:159-166.
PMid:26887239 PMCid:PMC4822756


17. Lopez BG, de Lourenço CC, Alves DA, Machado D, Lancellotti M, Sawaya AC. Antimicrobial and cytotoxic activity of red propolis: an alert for its safe use. J Appl Microbiol. 2015;119:677-87.


18. Martins ML, Leite KLF, Pacheco-Filho EF, Pereira AFM, Romanos MTV, Maia LC, et al. Efficacy of red propolis hydro-alcoholic extract in controlling Streptococcus mutans biofilm build-up and dental enamel demineralization. Arch Oral Biol. 2018;93:56-65.


19. Pippi B, Lana AJ, Moraes RC, Güez CM, Machado M, de Oliveira LF, et al. In vitro evaluation of the acquisition of resistance, antifungal activity and synergism of Brazilian red propolis with antifungal drugs on Candida spp. J Appl Microbiol. 2015;118:839-850.


20. Clinical and Laboratory Standards Institute (CLSI). Reference Method for Broth Dilution Antifungal Susceptibily Testing of Yeasts; Approved Standard - Third Edition. CLSI document M27-A3. Wayne PA, 2008.


21. Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007;42:321-424.
PMid:17560319 PMCid:PMC1895922


22. Pozzatti P, Loreto ES, Lopes PG, Athayde ML, Santurio JM, Alves SH. Comparison of the susceptibilities of clinical isolates of Candida albicans and Candida dubliniensis to essential oils. Mycoses. 2010;53:12-15.


23. Amaechi BT, Higham SM, Edgar WM. Techniques for the production of dental eroded lesions in vitro. J Oral Rehabil. 1999;26:97-102.


24. Loures FV, Levitz SM. XTT Assay of Antifungal Activity. Bio Protoc. 2015;5:e1543.
PMid:27453914 PMCid:PMC4957650


25. Holetz FB, Pessini GL, Sanches NR, Cortez DA, Nakamura CV, Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz. 2002;97:1027-1031.


26. Rabin N, Zheng Y, Opoku-Temeng C, Du Y, Bonsu E, Sintim HO. Biofilm formation mechanisms and targets for developing anti-biofilm agents. Future Med Chem 2015;7:493-512.


27. Ferreira GL, Pérez AL, Rocha IM, Pinheiro MA, de Castro RD, Carlo HL, et al. Does scientific evidence for the use of natural products in the treatment of oral candidiasis exist? A systematic review. Evid Based Complement Alternat Med. 2015;2015:147804.
PMid:25883668 PMCid:PMC4391323


28. Fürst R, Zündorf I. Evidence-Based Phytotherapy in Europe: Where Do We Stand? Planta Med. 2015;81:962-967.


29. Celerino de Moraes Porto IC, Chaves Cardoso de Almeida D, Vasconcelos Calheiros de Oliveira Costa G, Sampaio Donato TS, Moreira Nunes L, Gomes do Nascimento T, et al. Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent. BMC Complement Altern Med. 2018;18:219.
PMid:30021632 PMCid:PMC6052596


30. Oliveira JA, da Silva IC, Trindade LA, Lima EO, Carlo HL, Cavalcanti AL, et al. Safety and Tolerability of Essential Oil from Cinnamomum zeylanicum Blume Leaves with Action on Oral Candidosis and Its Effect on the Physical Properties of the Acrylic Resin. Evid Based Complement Alternat Med. 2014;2014:325670.
PMid:25574178 PMCid:PMC4276295