Chaves-Quirós C, Usuga-Usuga JS, Morales-Uchima SM, Tofiño-Rivera AP, Tobón-Arroyave SI, Martínez-Pabón MC. Assessment of cytotoxic and antimicrobial activities of two components of Cymbopogon citratus essential oil. J Clin Exp Dent. 2020;12(8):e749-54.

 

doi:10.4317/jced.56863

https://doi.org/10.4317/jced.56863

_______________________

 

References

1. Van der Weijden FA, Slot DE. Efficacy of homecare regimens for mechanical plaque removal in managing gingivitis a meta review. J Clin Periodontol. 2015;42:S77-91.
https://doi.org/10.1111/jcpe.12359
PMid:25597787

 

2. Sentila R, Gandhimathi A, Karthika S, Suryalakshmi R, Michael A. In-vitro evaluation and comparison of the anti-microbial potency of commercially available oral hygiene products against Streptococcus mutans. Indian J Med Sci. 2011;65:250-9.
https://doi.org/10.4103/0019-5359.107026
PMid:23391834

 

3. Faria G, Celes MR, De Rossi A, Silva LA, Silva JS, Rossi MA. Evaluation of chlorhexidine toxicity injected in the paw of mice and added to cultured l929 fibroblasts. J Endod. 2007;33:715-22.
https://doi.org/10.1016/j.joen.2006.12.023
PMid:17509413

 

4. Flemingson, Emmadi P, Ambalavanan N, Ramakrishnan T, Vijayalakshmi R. Effect of three commercial mouth rinses on cultured human gingival fibroblast: an in vitro study. Indian J Dent Res. 2008;19:29-35.
https://doi.org/10.4103/0970-9290.38929
PMid:18245921

 

5. Rajabalian S, Mohammadi M, Mozaffari B. Cytotoxicity evaluation of Persica mouthwash on cultured human and mouse cell lines in the presence and absence of fetal calf serum. Indian J Dent Res. 2009;20:169-73.
https://doi.org/10.4103/0970-9290.52894
PMid:19553717

 

6. Persson GR, Yeates J, Persson RE, Hirschi-Imfeld R, Weibel M, Kiyak HA. The impact of a low-frequency chlorhexidine rinsing schedule on the subgingival microbiota (the TEETH clinical trial). J Periodontol. 2007;78:1751-8.
https://doi.org/10.1902/jop.2007.070138
PMid:17760545

 

7. da Costa LFNP, Amaral CDSF, Barbirato DDS, Leão ATT, Fogacci MF. Chlorhexidine mouthwash as an adjunct to mechanical therapy in chronic periodontitis: A meta-analysis. J Am Dent Assoc. 2017;148:308-18.
https://doi.org/10.1016/j.adaj.2017.01.021
PMid:28284417

 

8. James P, Worthington HV, Parnell C, Harding M, Lamont T, Cheung A, et al. Chlorhexidine mouthrinse as an adjunctive treatment for gingival health. Cochrane Database Syst Rev. 2017; 3:CD008676.
https://doi.org/10.1002/14651858.CD008676.pub2
PMCid:PMC6464488

 

9. John G, Becker J, Schwarz F. Effects of taurolidine and chlorhexidine on SaOS-2 cells and human gingival fibroblasts grown on implant surfaces. Int J Oral Maxillofac Implants. 2014;29:728-34.
https://doi.org/10.11607/jomi.2956
PMid:24818214

 

10. Mariotti AJ, Rumpf DA. Chlorhexidine-induced changes to human gingival fibroblast collagen and non-collagen protein production. J Periodontol. 1999;70:1443-8.
https://doi.org/10.1902/jop.1999.70.12.1443
PMid:10632519

 

11. Moran J, Addy M, Roberts S. A comparison of natural product, triclosan and chlorhexidine mouthrinses on 4-day plaque regrowth. J Clin Periodontol. 1992;19:578-82.
https://doi.org/10.1111/j.1600-051X.1992.tb00686.x
PMid:1447382

 

12. Bernardes WA, Lucarini R, Tozatti MG, Flauzino LG, Souza MG, Turatti IC, et al. Antibacterial activity of the essential oil from Rosmarinus officinalis and its major components against oral pathogens. Z Naturforsch C. 2010;65:588-93.
https://doi.org/10.1515/znc-2010-9-1009
PMid:21138060

 

13. Ocheng F, Bwanga F, Joloba M, Softrata A, Azeem M, Pütsep K, et al. Essential oils from Ugandan aromatic medicinal plants: chemical composition and growth inhibitory effects on oral pathogens. Evid Based Complement Alternat Med. 2015;2015:230832.
https://doi.org/10.1155/2015/230832
PMid:26170872 PMCid:PMC4478384

 

14. Tofiño-Rivera A, Ortega-Cuadros M, Galvis-Pareja D, Jiménez-Rios H, Merini LJ, Martínez-Pabón MC. Effect of Lippia Alba and Cymbopogon Citratus essential oils on biofilms of Streptococcus Mutans and cytotoxicity in CHO cells. J Ethnopharmacol. 2016;194:749-54.
https://doi.org/10.1016/j.jep.2016.10.044
PMid:27765606

 

15. Oliveira MAC, Borges AC, Brighenti FL, Salvador MJ, Gontijo AVL, Koga-Ito CY. Cymbopogon citratus essential oil: effect on polymicrobial caries-related biofilm with low cytotoxicity. Braz Oral Res. 2017;31:e89.
https://doi.org/10.1590/1807-3107bor-2017.vol31.0089

PMid: 29116300

 

16. Ortega-Cuadros M, Tofiño-Rivera A, Merini LJ, Martínez-Pabón MC. Antimicrobial activity of Cymbopogon citratus (Poaceae) on Streptococcus mutans biofilm and its cytotoxic effects. Int J Trop Biol. 2018;66:1519-29.
https://doi.org/10.15517/rbt.v66i4.33140

PMid: 29116300

 

17. Almeida RBA, Akisue GII, Cardoso LMLII, Junqueira JCI, Jorge AOC. Antimicrobial activity of the essential oil of Cymbopogon citratus (DC) Stapf on Staphylococcus spp, Streptococcus mutans and Candida spp. Rev Bras Plantas Med. 2013;15:474-82.
https://doi.org/10.1590/S1516-05722013000400002

 

18. Onawunmi GO, Yisak WA, Ogunlana EO. Antibacterial constituents in the essential oil of Cymbopogon citratus (DC.) Stapf. J Ethnopharmacol. 1984;12:279-86.
https://doi.org/10.1016/0378-8741(84)90057-6

PMid: 6442749

 

19. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55-63.
https://doi.org/10.1016/0022-1759(83)90303-4

PMid: 6606682

 

20. Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods. 1986;89:271-7.
https://doi.org/10.1016/0022-1759(86)90368-6

PMid: 3486233

 

21. Sebaugh JL. Guidelines for accurate EC50/IC50 estimation. Pharm Stat. 2011;10:128-34.
https://doi.org/10.1002/pst.426
PMid:22328315

 

22. Rincón-Rodríguez RJ, Parada-Sanchez MT, Bedoya-Correa CM, Arboleda-Toro D. Genetic diversity of Streptococcus mutans serotype c isolated from white spot and cavitated caries lesions from schoolchildren. Arch Oral Biol. 2019;100:33-41.
https://doi.org/10.1016/j.archoralbio.2019.02.003
PMid:30776704

 

23. Fine DH, Furgang D, Barnett ML, Drew C, Steinberg L, Charles CH, et al. Effect of an essential oil-containing antiseptic mouthrinse on plaque and salivary Streptococcus mutans levels. J Clin Periodontol 2000;27:157-61.
https://doi.org/10.1034/j.1600-051x.2000.027003157.x
PMid:10743861

 

24. Giannelli M, Chellini F, Margheri M, Tonelli P, Tani A. Effect of chlorhexidine digluconate on different cell types: a molecular and ultrastructural investigation. Toxicol in Vitro. 2008;22:308-17.
https://doi.org/10.1016/j.tiv.2007.09.012
PMid:17981006

 

25. Wyganowska-Swiatkowska M, Urbaniak P, Szkaradkiewicz A, Jankun J, Kotwicka M. Effects of chlorhexidine, essential oils and herbal medicines (salvia, chamomile, calendula) on human fibroblast in vitro. Cent Eur J Immunol. 2016;41:125-31.
https://doi.org/10.5114/ceji.2016.59739
PMid:27536196 PMCid:PMC4967643

 

26. Eren K, Ozmeriç N, Sardaş S. Monitoring of buccal epithelial cells by alkaline comet assay (single cell gel electrophoresis technique) in cytogenetic evaluation of chlorhexidine. Clin Oral Investig. 2002;6:150-4.
https://doi.org/10.1007/s00784-002-0168-1
PMid:12271347

 

27. Ghabanchi J, Moattari A, Darafshi R, Andisheh Tadbir A, Khorshidi H, Shakib M. Effects of three commercial mouth rinses on the cultured fibroblasts: an in vitro study. J Dent (Shiraz). 2013;14:64-7.

PMid: 24724121 PMCid: PMC3977549

 

28. Vasireddy L, Bingle LEH, Davies MS. Antimicrobial activity of essential oils against multidrug-resistant clinical isolates of the Burkholderia cepacia complex. PLoS One. 2018;13:e0201835.
https://doi.org/10.1371/journal.pone.0201835
PMid:30071118 PMCid:PMC6072103

 

29. Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils--a review. Food Chem Toxicol. 2008;46:446-75.
https://doi.org/10.1016/j.fct.2007.09.106
PMid:17996351

 

30. Dorman HJ, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol. 2000;88:308-16.
https://doi.org/10.1046/j.1365-2672.2000.00969.x
PMid:10736000

 

31. Yoon HS, Moon SC, Kim ND, Park BS, Jeong MH, Yoo YH. Genistein induces apoptosis of RPE-J cells by opening mitochondrial PTP. Biochem Biophys Res Commun. 2000;276:151-6.
https://doi.org/10.1006/bbrc.2000.3445
PMid:11006098

 

32. Armstrong JS. Mitochondrial membrane permeabilization: the sine qua non for cell death. Bioessays. 2006;28:253-60.
https://doi.org/10.1002/bies.20370
PMid:16479581

 

33. Paula VA, Modesto A, Santos KR, Gleiser R. Antimicrobial effects of the combination of chlorhexidine and xylitol. Br Dent J. 2010;209:E19.
https://doi.org/10.1038/sj.bdj.2010.887
PMid:20885412

 

34. Shunying Z, Yang Y, Huaidong Y, Yue Y, Guolin Z. Chemical composition and antimicrobial activity of the essential oils of Chrysanthemum indicum. J Ethnopharmacol. 2005;96:151-8.
https://doi.org/10.1016/j.jep.2004.08.031
PMid:15588664

 

35. Pankey GA, Sabath LD. Clinical relevance of bacteriostatic versus bactericidal mechanisms of action in the treatment of Gram-positive bacterial infections. Clin Infect Dis. 2004;38:864-70.
https://doi.org/10.1086/381972
PMid:14999632