Palaia G, Mohsen M, Pergolini D, Bartone V, Purrazzella A, Romeo U, Polimeni A. E-cigarette: a safe tool or a risk factor for oral cancer? A systematic review. J Clin Exp Dent. 2025;17(2):e219-28.

 

doi:10.4317/jced.62449

https://doi.org/10.4317/jced.62449

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References

1. Marques P, Piqueras L, Sanz MJ. An updated overview of e cigarette impact on human health", Marques et al. Respir Res. 2021;22(1):151.
https://doi.org/10.1186/s12931-021-01737-5
PMid:34006276 PMCid:PMC8129966

 

2. Guo J, Hecht SS. DNA damage in human oral cells induced by use of e-cigarettes. Drug Test Anal. 2023;15(10):1189-1197.
https://doi.org/10.1002/dta.3375
PMid:36169810 PMCid:PMC10043052

 

3. Sancilio S, Gallorini M, Cataldi A, di Giacomo V. Cytotoxicity and apoptosis induction by e-cigarette fluids in human gingival fibroblasts. Clin Oral Investig. 2016;20(3):477-83.
https://doi.org/10.1007/s00784-015-1537-x
PMid:26239821

 

4. Farsalinos KE, Romagna G, Tsiapras D, Kyrzopoulos S, Voudris V. Characteristics, perceived side effects and benefits of electronic cigarette use: a worldwide survey of more than 19,000 consumers. Int J Environ Res Public Health. 2014;11(4):4356-73.
https://doi.org/10.3390/ijerph110404356
PMid:24758891 PMCid:PMC4025024

 

5. Selya AS, Dierker L, Rose JS, Hedeker D, Mermelstein RJ. The Role of Nicotine Dependence in E-Cigarettes' Potential for Smoking Reduction. Nicotine Tob Res. 2018;20(10):1272-1277.
https://doi.org/10.1093/ntr/ntx160
PMid:29065204 PMCid:PMC6121914

 

6. Javed F, Kellesarian SV, Sundar IK, Romanos GE, Rahman I. Recent updates on electronic cigarette aerosol and inhaled nicotine effects on periodontal and pulmonary tissues. Oral Dis. 2017;23(8):1052-1057.
https://doi.org/10.1111/odi.12652
PMid:28168771 PMCid:PMC5545167

 

7. Bardellini E, Amadori F, Conti G, Majorana A. Oral mucosal lesions in electronic cigarettes consumers versus former smokers. Acta Odontol Scand. 2018;76(3):226- 228.
https://doi.org/10.1080/00016357.2017.1406613
PMid:29161938

 

8. Willershausen I, Wolf T, Weyer V, Sader R, Ghanaati S, Willershausen B. Influence of E-smoking liquids on human periodontal ligament fibroblasts. Head Face Med. 2014;10:39.
https://doi.org/10.1186/1746-160X-10-39
PMid:25224853 PMCid:PMC4237808

 

9. Brooks JK, Kleinman JW, Brooks JB, Reynolds MA. Electronic cigarette explosion associated with extensive intraoral injuries. Dent Traumatol. 2017;33(2):149-152.
https://doi.org/10.1111/edt.12293
PMid:27397137

 

10. Golbach LA, Portelli LA, Savelkoul HFJ, Terwel SR, Kuster N, de Vries RBM, et al. Calcium homeostasis and low-frequency magnetic and electric field expo-sure: A systematic review and meta-analysis of in vitro studies. Environ. Int. 2016;92-93:695-706.
https://doi.org/10.1016/j.envint.2016.01.014
PMid:26872872

 

11. Slim K, Nini E, Forestier D, Kwiatkowski F, Panis Y, Chipponi J. Methodological index for non-randomized studies (minors): Development and validation of a new instrument. ANZ J. Surg. 2003;73:712-716.
https://doi.org/10.1046/j.1445-2197.2003.02748.x
PMid:12956787

 

12. Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al. The PRISMA 2020 statement: An updated guideline for reporting systematic reviews. BMJ. 2021;372:n71.
https://doi.org/10.1136/bmj.n71
PMid:33782057 PMCid:PMC8005924

 

13. Ye Dongxia, Gajendra S, Lawyer G, Jadeja N, Pishey D, Pathagunti S, et al. Inflammatory biomarkers and growth factors in saliva and gingival crevicular fluid of e-cigarette users, cigarette smokers, and dual smokers: A pilot study. J Periodontol. 2020;91(10):1274-1283.
https://doi.org/10.1002/JPER.19-0457
PMid:32052441 PMCid:PMC9014419

 

14. Kamal NM, Shams NS. The impact of tobacco smoking and electronic cigarette vaping on salivary biomarkers: A comparative study. Saudi Dent J. 2022;34(5):404-409.
https://doi.org/10.1016/j.sdentj.2022.05.003
PMid:35814841 PMCid:PMC9263753

 

15. Tommasi S, Caliri AW, Caceres A, Moreno DE, Li M, Chen Y, et al. Deregulation of biologically significant genes and associated molecular pathways in the oral epithelium of electronic cigarette users. Int J Mol Sci. 2019;20(3):738.
https://doi.org/10.3390/ijms20030738
PMid:30744164 PMCid:PMC6386888

 

16. Vermehren MF, Wiesmann N, Deschner J, Brieger J, Al-Nawas B, Kämmerer PW. Comparative analysis of the impact of e-cigarette vapor and cigarette smoke on human gingival fibroblasts. Toxicol In Vitro. 2020;69:105005.
https://doi.org/10.1016/j.tiv.2020.105005
PMid:32956835

 

17. Yu V, Rahimy M, Korrapati A, Xuan Y, Zou AE, Krishnan AR, et al. Electronic cigarettes induce DNA strand breaks and cell death independently of nicotine in cell lines. Oral Oncol. 2016;52:58-65.
https://doi.org/10.1016/j.oraloncology.2015.10.018
PMid:26547127 PMCid:PMC4891196

 

18. De Lima JM, Macedo CCS, Barbosa GV, Castellano LRC, Hier MP, Alaoui-Jamali MA, et al. E-liquid alters oral epithelial cell function to promote epithelial to mesenchymal transition and invasiveness in preclinical oral squamous cell carcinoma. Sci Rep. 2023;13:3330.
https://doi.org/10.1038/s41598-023-30016-0
PMid:36849550 PMCid:PMC9971414

 

19. Wisniewski DJ, Ma T, Schneider A. Nicotine induces oral dysplastic keratinocyte migration via fatty acid syn-thase-dependent epidermal growth factor receptor activation. Exp. Cell Res. 2018;370:343-352.
https://doi.org/10.1016/j.yexcr.2018.06.036
PMid:29966661 PMCid:PMC6108942

 

20. Schwarzmeier LAT, da Cruz BS, Ferreira CCP, Carvalho BFC, Alves MGO, Carta CFL, et al. E-cig might cause cell damage of oral mucosa. Oral Surg Oral Med Oral Pathol Oral Radiol. 2021;131(4):435-443.

PMid:33610539

 

21. Pandarathodiyil AK, Ramanathan A, Garg R, Doss JG, Abd Rahman FB, Ghani WMN, et al. Lactate Dehydrogenase Levels in the Saliva of Cigarette and E-Cigarette Smokers (Vapers): A Comparative Analysis. Asian Pac J Cancer Prev. 2021;22(10):3227-3235.
https://doi.org/10.31557/APJCP.2021.22.10.3227
PMid:34710999 PMCid:PMC8858253

 

22. Hamad SH, Brinkman MC, Tsai YH, Mellouk N, Cross K, Jaspers I, et al. Pilot Study to Detect Genes Involved in DNA Damage and Cancer in Humans: Potential Biomarkers of Exposure to E-Cigarette Aerosols. Genes (Basel). 2021;12(3):448.
https://doi.org/10.3390/genes12030448
PMid:33809907 PMCid:PMC8004185

 

23. Rogér JM, Abayon M, Elad S, Kolokythas A. Oral Trauma and Tooth Avulsion Following Explosion of E-Cigarette. J Oral Maxillofac Surg. 2016;74(6):1181-5.
https://doi.org/10.1016/j.joms.2015.12.017
PMid:26850869

 

24. Harrison R, Hicklin D Jr. Electronic cigarette explosions involving the oral cavity. J Am Dent Assoc. 2016;147(11):891-896.
https://doi.org/10.1016/j.adaj.2016.03.018
PMid:27158079

 

25. Goniewicz ML, Knysak J, Gawron M, Kosmider L, Sobczak A, Kurek J, et al. Levels of selected carcinogens and toxicants in vapour from electronic cigarettes. Tob Control. 2014;23(2):133-139.
https://doi.org/10.1136/tobaccocontrol-2012-050859
PMid:23467656 PMCid:PMC4154473

 

26. Sundar IK, Javed F, Romanos GE, Rahman I. E-cigarettes and flavorings induce inflammatory and pro-senescence responses in oral epithelial cells and periodontal fibroblasts. Oncotarget. 2016;7(47):77196-77204.
https://doi.org/10.18632/oncotarget.12857
PMid:27791204 PMCid:PMC5363580

 

27. Yao H, Edirisinghe I, Rajendrasozhan S, Yang SR, Caito S, Adenuga D, et al. Cigarette smoke-mediated inflammatory and oxidative responses are strain-dependent in mice. Am J Physiol Lung Cell Mol Physiol. 2008;294:L1174-L1186.
https://doi.org/10.1152/ajplung.00439.2007
PMid:18375740

 

28. Pop AM, Coros R, Stoica AM, Monea M. Early diagnosis of oral mucosal alterations in smokers and e-cigarette users based on micronuclei count: a cross-sectional study among dental students. Int J Environ Res Public Health. 2021;18(24):13246.
https://doi.org/10.3390/ijerph182413246
PMid:34948855 PMCid:PMC8707162

 

29. Fuller TW, Acharya AP, Meyyappan T, et al. Comparison of Bladder Carcinogens in the Urine of E-cigarette Users Versus Non E-cigarette Using Controls. Sci Rep. 2018;8(1):507.
https://doi.org/10.1038/s41598-017-19030-1
PMid:29323232 PMCid:PMC5765148

 

30. Ramoa CP, Eissenberg T, Sahingur SE. Increasing popularity of waterpipe tobacco smoking and electronic cigarette use: Implications for oral healthcare. J Periodontal Res. 2017;52(5):813-823.
https://doi.org/10.1111/jre.12458
PMid:28393367 PMCid:PMC5585021

 

31. Tsai KYF, Hirschi Budge KM, Lepre AP, Rhees MS, Ajdaharian J, Geiler J, et al. Cell invasion, RAGE expression, and inflammation in oral squamous cell carcinoma (OSCC) cells exposed to e-cigarette flavoring. Clin Exp Dent Res. 2020;6:618-625.
https://doi.org/10.1002/cre2.314
PMid:32783323 PMCid:PMC7745078

 

32. Fagan P, Pokhrel P, Herzog TA, Moolchan ET, Cassel KD, Franke AA, et al. Addictive Carcinogens Workgroup. Sugar and Aldehyde Content in Flavored Electronic Cigarette Liquids. Nicotine Tob Res. 2018;20(8):985-992.
https://doi.org/10.1093/ntr/ntx234
PMid:29182761 PMCid:PMC6037055

 

33. Hajek P, Phillips-Waller A, Przulj D, Pesola F, Myers Smith K, Bisal N, et al. A Randomized Trial of E-Cigarettes versus Nicotine-Replacement Therapy. N Engl J Med. 2019;380(7):629-637.
https://doi.org/10.1056/NEJMoa1808779
PMid:30699054

 

34. Canistro D, Vivarelli F, Cirillo S, Babot Marquillas C, Buschini A, Lazzaretti M, et al. E-cigarettes induce toxicological effects that can raise the cancer risk. Sci Rep. 2017;7(1):2028.
https://doi.org/10.1038/s41598-017-02317-8
PMid:28515485 PMCid:PMC5435699

 

35. Klawinski D, Hanna I, Breslin NK, Katzenstein HM, Indelicato DJ. Vaping the Venom: Oral Cancer in a Young Adult With Extensive Electronic Cigarette Use. Pediatrics. 2021;147(5):e2020022301.
https://doi.org/10.1542/peds.2020-022301
PMid:33926987

 

36. Pergolini D, Botticelli A, Fascetti R, Rocchetti F, Cirillo A, Tenore G, et al. Oral Immune-Related Adverse Events Associated with PD-1 Inhibitor Treatment: A Case Series. Applied Sciences. 2022;12(24):12994.
https://doi.org/10.3390/app122412994

 

37. Kim SM, Hwang KA, Choi DW, Choi KC. The cigarette smoke components induced the cell proliferation and epithelial to mesenchymal transition via production of reactive oxygen species in endometrial adenocarcinoma cells. Food Chem Toxicol. 2018;121:657-665.
https://doi.org/10.1016/j.fct.2018.09.023
PMid:30236600

 

38. Welz C, Canis M, Schwenk-Zieger S, Becker S, Stucke V, Ihler F, et al. toxic and Genotoxic Effects of Electronic Cigarette Liquids on Human Mucosal Tissue Cultures of the Oropharynx. J Environ Pathol Toxicol Oncol. 2016;35(4):343-354.
https://doi.org/10.1615/JEnvironPatholToxicolOncol.2016016652
PMid:27992314