Firouzi N, Asheghi B, Chitsazha R, Ayoobi M, Mazidi MMK, Pourhajibagher M. A comprehensive evaluation of the antimicrobial efficacy of concentrated growth factor against biofilms associated with endodontic diseases: An in vitro study. J Clin Exp Dent. 2026;18(2):e241-9.

 

doi:10.4317/jced.63049

https://doi.org/10.4317/jced.63049

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References

1. Kim SG, Malek M, Sigurdsson A, Lin LM, Kahler B. Regenerative endodontics: a comprehensive review. Int Endod J. 2018;51(12):1367-1388.
https://doi.org/10.1111/iej.12954
PMid:29777616

 

2. Demarco FF, Conde MC, Cavalcanti BN, Casagrande L, Sakai VT, Nör JE. Dental pulp tissue engineering. Braz Dent J. 2011;22(1):3-13.
https://doi.org/10.1590/S0103-64402011000100001
PMid:21519641 PMCid:PMC3736569

 

3. Galler KM. Clinical procedures for revitalization: current knowledge and considerations. Int Endod J. 2016;49(10):926-936.
https://doi.org/10.1111/iej.12606
PMid:26715631

 

4. Yan H, De-Deus G, Kristoffersen IM, Wiig E, Reseland JE, Johnsen GF, et al. Regenerative endodontics by cell homing: a review of recent clinical trials. J Endod. 2023;49(1):4-17.
https://doi.org/10.1016/j.joen.2022.09.008
PMid:36270575

 

5. Sabeti MA, Saqib Ihsan M, Adami D, Hassani SN, Moushekhian S, Shafieian R, et al. Cell-based regenerative endodontics for the treatment of irreversible pulpitis: an in vivo investigation. J Endod. 2024;50(3):344-350.
https://doi.org/10.1016/j.joen.2023.11.014
PMid:38142887

 

6. Dhillon H, Kaushik M, Sharma R. Regenerative endodontics-creating new horizons. J Biomed Mater Res B Appl Biomater. 2016;104(4):676-685.
https://doi.org/10.1002/jbm.b.33587
PMid:26699211

 

7. Antunes LS, Salles AG, Gomes CC, Andrade TB, Delmindo MP, Antunes LA. The effectiveness of pulp revascularization in root formation of necrotic immature permanent teeth: a systematic review. Acta Odontol Scand. 2016;74(3):161-169.
https://doi.org/10.3109/00016357.2015.1069394
PMid:26179397

 

8. Jiang X, Liu H. The capability of regenerative endodontics to promote the complete root formation in immature permanent teeth with different initial pre-operative conditions. Clin Oral Investig. 2023;28(1):6.
https://doi.org/10.1007/s00784-023-05403-6
PMid:38123737

 

9. Chávez de Paz LE, Bergenholtz G, Svensäter G. The effects of antimicrobials on endodontic biofilm bacteria. J Endod. 2010;36(1):70-77.
https://doi.org/10.1016/j.joen.2009.09.017
PMid:20003938

 

10. Gomes B, Aveiro E, Kishen A. Irrigants and irrigation activation systems in endodontics. Braz Dent J. 2023;34(4):1-33.
https://doi.org/10.1590/0103-6440202305577

 

11. Huang GT. A paradigm shift in endodontic management of immature teeth: conservation of stem cells for regeneration. J Dent. 2008;36(6):379-386.
https://doi.org/10.1016/j.jdent.2008.03.002
PMid:18420332

 

12. Velmurugan N, Sooriaprakas C, Jain P. Apical extrusion of irrigants in immature permanent teeth by using EndoVac and needle irrigation: an in vitro study. J Dent (Tehran). 2014;11(4):433-439.

 

13. Namour M, Theys S. Pulp revascularization of immature permanent teeth: a review of the literature and a proposal of a new clinical protocol. ScientificWorldJournal. 2014;2014:737503.
https://doi.org/10.1155/2014/737503
PMid:25383384 PMCid:PMC4212590

 

14. Wang L, Wan M, Li Z, Zhong N, Liang D, Ge L. A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro. Mol Med Rep. 2019;20(2):1039-1048.
https://doi.org/10.3892/mmr.2019.10313
PMid:31173196 PMCid:PMC6625392

 

15. Alauddin MS, Yusof NM, Adnan AS, Said Z. Preliminary novel analysis on antimicrobial properties of concentrated growth factor against bacteria-induced oral diseases. Eur J Dent. 2022;16(4):901-910.
https://doi.org/10.1055/s-0041-1742121
PMid:35189641 PMCid:PMC9683896

 

16. Drago L, Bortolin M, Vassena C, Taschieri S, Del Fabbro M. Antimicrobial activity of pure platelet-rich plasma against microorganisms isolated from oral cavity. BMC Microbiol. 2013;13:47.
https://doi.org/10.1186/1471-2180-13-47
PMid:23442413 PMCid:PMC3599521

 

17. Melo-Ferraz A, Coelho C, Miller P, Criado MB, Monteiro MC. Platelet activation and antimicrobial activity of L-PRF: a preliminary study. Mol Biol Rep. 2021;48(5):4573-4580.
https://doi.org/10.1007/s11033-021-06487-7
PMid:34146200

 

18. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. CLSI supplement M100. 30th ed. Wayne, PA: CLSI; 2020.

 

19. Macià MD, Rojo-Molinero E, Oliver A. Antimicrobial susceptibility testing in biofilm-growing bacteria. Clin Microbiol Infect. 2014;20(10):981-990.
https://doi.org/10.1111/1469-0691.12651
PMid:24766583

 

20. Ceri H, Olson ME, Stremick C, Read RR, Morck D, Buret A. The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. J Clin Microbiol. 1999;37(6):1771-1776.
https://doi.org/10.1128/JCM.37.6.1771-1776.1999
PMid:10325322 PMCid:PMC84946

 

21. Miles AA, Misra SS, Irwin JO. The estimation of the bactericidal power of the blood. J Hyg (Lond). 1938;38(6):732-749.
https://doi.org/10.1017/S002217240001158X
PMid:20475467 PMCid:PMC2199673

 

22. Yoo YJ, Kim AR, Perinpanayagam H, Han SH, Kum KY. Candida albicans virulence factors and pathogenicity for endodontic infections. Microorganisms. 2020;8(9):1418.
https://doi.org/10.3390/microorganisms8091300
PMid:32858856 PMCid:PMC7563224

 

23. Ramage G, Saville SP, Thomas DP, López-Ribot JL. Candida biofilms: an update. Eukaryot Cell. 2005;4(4):633-638.
https://doi.org/10.1128/EC.4.4.633-638.2005
PMid:15821123 PMCid:PMC1087806

 

24. Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J. 2003;36(1):1-11.
https://doi.org/10.1046/j.1365-2591.2003.00603.x
PMid:12656508

 

25. Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J. 2002;35(3):221-228.
https://doi.org/10.1046/j.1365-2591.2002.00504.x
PMid:11985673

 

26. Ding RY, Cheung GS, Chen J, Yin XZ, Wang QQ, Zhang CF. Pulp revascularization of immature teeth with apical periodontitis: a clinical study. J Endod. 2009;35(5):745-749.
https://doi.org/10.1016/j.joen.2009.02.009
PMid:19410097

 

27. Yoshpe M, Ruparel N, Einy S, Ganatra S, Kaufman AY. Treatment of necrotic anterior and posterior teeth with regenerative endodontic procedures using PRF as a scaffold: a retrospective study. Appl Sci. 2022;12(13):6774.
https://doi.org/10.3390/app12136774

 

28. Jacobs JC, Troxel A, Ehrlich Y, Spolnik K, Bringas JS, Gregory RL, et al. Antibacterial effects of antimicrobials used in regenerative endodontics against biofilm bacteria obtained from mature and immature teeth with necrotic pulps. J Endod. 2017;43(4):575-579.
https://doi.org/10.1016/j.joen.2016.12.014
PMid:28216269

 

29. Kim SG. Infection and pulp regeneration. Dent J (Basel). 2016;4(1):8.
https://doi.org/10.3390/dj4010004
PMid:29563446 PMCid:PMC5851207

 

30. Rahimi S, Janani M, Lotfi M, Shahi S, Aghbali A, Pakdel MV, et al. A review of antibacterial agents in endodontic treatment. Iran Endod J. 2014;9(3):161-168.

 

31. Mohammadi Z. Sodium hypochlorite in endodontics: an update review. Int Dent J. 2008;58(6):329-341.
https://doi.org/10.1111/j.1875-595X.2008.tb00354.x
PMid:19145794

 

32. Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, et al. Concentration-dependent effect of sodium hypochlorite on stem cells of apical papilla survival and differentiation. J Endod. 2014;40(1):51-55.
https://doi.org/10.1016/j.joen.2013.07.026
PMid:24331991

 

33. Kraemer BF, Campbell RA, Schwertz H, Cody MJ, Franks Z, Tolley ND, et al. Novel antibacterial activities of β-defensin 1 in human platelets: suppression of pathogen growth and signaling of neutrophil extracellular trap formation. PLoS Pathog. 2011;7(11):e1002355.
https://doi.org/10.1371/journal.ppat.1002355
PMid:22102811 PMCid:PMC3213094

 

34. Karan CL, Jeyaraman M, Jeyaraman N, Ramasubramanian S, Khanna M, Yadav S. Antimicrobial effects of platelet-rich plasma and platelet-rich fibrin: a scoping review. Cureus. 2023;15(12):e51360.

 

35. Feng M, Wang Y, Zhang P, Zhao Q, Yu S, Shen K, et al. Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation. Int J Oral Sci. 2020;12(1):32.
https://doi.org/10.1038/s41368-020-00099-w
PMid:33243983 PMCid:PMC7693325

 

36. Ghebrehiwet B, Kaplan AP, Joseph K, Peerschke EI. The complement and contact activation systems: partnership in pathogenesis beyond angioedema. Immunol Rev. 2016;274(1):281-289.
https://doi.org/10.1111/imr.12469
PMid:27782339

 

37. Firouzi N, Yavari HR, Rahimi S, Roshangar L, Chitsazha R, Amini M. Concentrated growth factors combined with lipopolysaccharide stimulate the in vitro regenerative and osteogenic activities of human dental pulp stem cells by balancing inflammation. Int J Dent. 2022;2022:2316666.
https://doi.org/10.1155/2022/2316666
PMid:36571070 PMCid:PMC9780000

 

38. Tian S, Wang J, Dong F, Du N, Li W, Song P, et al. Concentrated growth factor promotes dental pulp cells proliferation and mineralization and facilitates recovery of dental pulp tissue. Med Sci Monit. 2019;25:10016-10028.
https://doi.org/10.12659/MSM.919316
PMid:31877561 PMCid:PMC6944166

 

39. Chen X, Chen Y, Hou Y, Song P, Zhou M, Nie M, et al. Modulation of proliferation and differentiation of gingiva-derived mesenchymal stem cells by concentrated growth factors: potential implications in tissue engineering for dental regeneration and repair. Int J Mol Med. 2019;44(1):37-46.
https://doi.org/10.3892/ijmm.2019.4172
PMid:31017269 PMCid:PMC6559294

 

40. Luo H, Liu W, Zhou Y, Jiang X, Liu Y, Yang Q, et al. Concentrated growth factor regulates the macrophage-mediated immune response. Regen Biomater. 2021;8(6):rbab049.
https://doi.org/10.1093/rb/rbab049
PMid:34513006 PMCid:PMC8421811

 

41. Tang YQ, Yeaman MR, Selsted ME. Antimicrobial peptides from human platelets. Infect Immun. 2002;70(12):6524-6533.
https://doi.org/10.1128/IAI.70.12.6524-6533.2002
PMid:12438321 PMCid:PMC132966

 

42. Krijgsveld J, Zaat SA, Meeldijk J, van Veelen PA, Fang G, Poolman B, et al. Thrombocidins, microbicidal proteins from human blood platelets, are C-terminal deletion products of CXC chemokines. J Biol Chem. 2000;275(27):20374-20382.
https://doi.org/10.1074/jbc.275.27.20374
PMid:10877842

 

43. Coelho LAS, Bordini EAF, Cassiano FB, de Toledo Stuani V, Silva RM, dos Passos DGS, et al. Impact of endodontic irrigant solutions on the viability and metabolism of stem cells from apical papilla: proposal of a novel in vitro model. Clin Oral Investig. 2025;29(2):136-146.
https://doi.org/10.1007/s00784-025-06231-6
PMid:39964548