Dias MS, Furtado JLF, Damasceno MAF, de Carvalho JC, Gondim DV, Alcalde MP, Duarte MAH, de Vasconcelos BC. Bioactivity and dimensional stability of resin-, hybrid-, and silicate-based endodontic sealers: An in vitro comparative study. J Clin Exp Dent. 2026;18(5):e605-10.

doi:10.4317/jced.63855

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References

1. Zordan-Bronzel CL, Esteves Torres FF, Tanomaru-Filho M, Chávez-Andrade GM, Bosso-Martelo R, Guerreiro-Tanomaru JM. Evaluation of physicochemical properties of a new calcium silicate-based sealer, Bio-C Sealer. J Endod 2019;45:1248-1252. https://doi.org/10.1016/j.joen.2019.07.006 PMid:31447172

2. Silva EJ, Accorsi-Mendonça T, Pedrosa AC, Granjeiro JM, Zaia AA. Long-Term Cytotoxicity, pH and Dissolution Rate of AH Plus and MTA Fillapex. Braz Dent J 2016;27:419-423. https://doi.org/10.1590/0103-6440201600735 PMid:27652704

3. Ortega MA, Rios L, Fraile-Martinez O, Liviu Boaru D, De Leon-Oliva D, Barrena-Blázquez S, et al. Bioceramic versus traditional biomaterials for endodontic sealers according to the ideal properties. Histol Histopathol. 2024;39:279-292.

4. Fristad I, Haug S, Bårdsen A. Biological properties versus solubility of endodontic sealers and cements. Biomater Investig Dent 2024;11:40863. https://doi.org/10.2340/biid.v11.40863 PMid:38903777 PMCid:PMC11187977

5. Poggio C, Dagna A, Ceci M, Meravini MV, Colombo M, Pietrocola G. Solubility and pH of bioceramic root canal sealers: A comparative study. J Clin Exp Dent 2017;9:e1189-e1194.. https://doi.org/10.4317/jced.54040 PMid:29167707 PMCid:PMC5694146

6. Holland R, Gomes JE Filho, Cintra LTA, Queiroz ÍOA, Estrela C. Factors affecting the periapical healing process of endodontically treated teeth. J Appl Oral Sci 2017;25:465-476. https://doi.org/10.1590/1678-7757-2016-0464 PMid:29069143 PMCid:PMC5804382

7. Kalantar Motamedi MR, Mortaheb A, Zare Jahromi M, Gilbert BE. Micro-CT Evaluation of Four Root Canal Obturation Techniques. Scanning 2021;2021:6632822. https://doi.org/10.1155/2021/6632822 PMid:33717394 PMCid:PMC7932785

8. Bhandi S, Mashyakhy M, Abumelha AS, Alkahtany MF, Jamal M, Chohan H, et al. Complete obturation-cold lateral condensation vs. thermoplastic techniques: a systematic review of micro-ct studies. Materials (Basel) 2021;14:4013. https://doi.org/10.3390/ma14144013 PMid:34300930 PMCid:PMC8304925

9. Hachem CE, Chedid JCA, Nehme W, Kaloustian MK, Ghosn N, Sahnouni H, et al. Physicochemical and antibacterial properties of conventional and two premixed root canal filling materials in primary teeth. J Funct Biomater 2022;13:177. https://doi.org/10.3390/jfb13040177 PMid:36278646 PMCid:PMC9589963

10. Bhat A, Cvach N, Mizuno C, Ahn C, Zhu Q, Primus C, et al. Ion Release from prototype Surface Pre-Reacted Glass Ionomer (S-PRG) Sealer and EndoSequence BC Sealer. Eur Endod J 2021;6:122-127. https://doi.org/10.14744/eej.2020.50470 PMid:33762532 PMCid:PMC8056809

11. Estrela C, Cintra LTA, Duarte MAH, Rossi-Fedele G, Gavini G, Sousa-Neto MD. Mechanism of action of bioactive endodontic materials. Braz Dent J 2023;34:1-11. https://doi.org/10.1590/0103-6440202305278 PMid:36888836 PMCid:PMC10027099

12. Prasad Kumara PAAS, Cooper PR, Cathro P, Gould M, Dias G, Ratnayake J. Bioceramics in endodontics: limitations and future innovations-A Review. Dent J (Basel) 2025;13:157. https://doi.org/10.3390/dj13040157 PMid:40277487 PMCid:PMC12026347

13. Widbiller M, Schmalz G. Endodontic regeneration: hard shell, soft core. Odontology 2021;109:303-312. https://doi.org/10.1007/s10266-020-00573-1 PMid:33263826 PMCid:PMC7954765

14. Al-Haddad A, Che Ab Aziz ZA. Bioceramic-based root canal sealers: a review. Int J Biomater 2016; 2016:9753210. https://doi.org/10.1155/2016/9753210 PMid:27242904 PMCid:PMC4868912

15. Candeiro GTM, Lavor AB, Lima ITF, Vasconcelos BC, Gomes NV, Iglecias EF, et al. Penetration of bioceramic and epoxy-resin endodontic cements into lateral canals. Braz Oral Res 2019;33:e049. https://doi.org/10.1590/1807-3107bor-2019.vol33.0049 PMid:31141039

16. Dong X, Xu X. Bioceramics in endodontics: updates and future perspectives. Bioengineering (Basel) 2023;10:354. https://doi.org/10.3390/bioengineering10030354 PMid:36978746 PMCid:PMC10045528

17. Wannakajeepiboon M, Sathorn C, Kornsuthisopon C, Santiwong B, Wasanapiarnpong T, Linsuwanont P. Evaluation of the chemical, physical, and biological properties of a newly developed bioceramic cement derived from cockle shells: an in vitro study. BMC Oral Health 2023;23:354. https://doi.org/10.1186/s12903-023-03073-0 PMid:37270491 PMCid:PMC10239161

18. Nagendrababu V, Murray PE, Ordinola-Zapata R, Peters OA, Rôças IN, Siqueira JF Jr, et al. PRILE 2021 guidelines for reporting laboratory studies in Endodontology: a consensus-based development. Int Endod J 2021;54:1482-1490. https://doi.org/10.1111/iej.13542 PMid:33938010

19. de Vasconcelos BC, Bernardes RA, Cruz SM, Duarte MA, Padilha P de M, Bernardineli N, et al. Evaluation of pH and calcium ion release of new root-end filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:135-139. https://doi.org/10.1016/j.tripleo.2009.02.026 PMid:19451009

20. Souza LC, Neves GST, Kirkpatrick T, Letra A, Silva R. Physicochemical and biological properties of AH Plus Bioceramic. J Endod 2023;49:69-76. https://doi.org/10.1016/j.joen.2022.10.009 PMid:36279961

21. Lee JK, Kwak SW, Ha JH, Lee W, Kim HC. Physicochemical properties of epoxy resin-based and bioceramic-based root canal sealers. Bioinorg Chem Appl 2017;2017:2582849. https://doi.org/10.1155/2017/2582849 PMid:28210204 PMCid:PMC5292198

22. Kwak SW, Koo J, Song M, Jang IH, Gambarini G, Kim HC. Physicochemical properties and biocompatibility of various bioceramic root canal sealers: in vitro study. J Endod 2023;49:871-879. https://doi.org/10.1016/j.joen.2023.05.013 PMid:37225037

23. Ashraf H, Mortezapour N, Jabari S, Zadsirjan S, Tabatabai FS. Evaluation of chemical and physical properties of an experimental endodontic sealer in comparison with AH-26 and AH-Plus. Iran Endod J 2020;15:183-187.

24. Giraud T, Jeanneau C, Rombouts C, Bakhtiar H, Laurent P, About I. Pulp capping materials modulate the balance between inflammation and regeneration. Dent Mater. 2019 Jan;35(1):24-35. https://doi.org/10.1016/j.dental.2018.09.008 PMid:30269862

25. Al-Sherbiny IM, Farid MH, Abu-Seida AM, Motawea IT, Bastawy HA. Chemico-physical and mechanical evaluation of three calcium silicate-based pulp capping materials. Saudi Dent J 2021;33:207-214. https://doi.org/10.1016/j.sdentj.2020.02.001 PMid:34025083 PMCid:PMC8119770

26. Quaresma SAL, Alves Dos Santos GN, Silva-Sousa AC, Camargo RV, Lopes-Olhê FC, Silva-Sousa YTC, et al. Physicochemical properties of calcium silicate cement based endodontic sealers. J Mech Behav Biomed Mater 2024;151:106400. https://doi.org/10.1016/j.jmbbm.2024.106400 PMid:38262184

27. Belal RS, Edanami N, Yoshiba K, Yoshiba N, Ohkura N, Takenaka S, et al. Comparison of calcium and hydroxyl ion release ability and in vivo apatite-forming ability of three bioceramic-containing root canal sealers. Clin Oral Investig 2022;26:1443-1451. https://doi.org/10.1007/s00784-021-04118-w PMid:34398328

28. Pérez-Guzmán N, García-Rios P, Rodríguez-Lozano FJ, García-Bernal D, El Yahyaoui A, López-García S. Ultrastructural and immunobiological responses of human periodontal ligament stem cells to novel tricalcium silicate sealers. Dent Mater. 2026 Feb;42(2):331-341. https://doi.org/10.1016/j.dental.2025.10.011 PMid:41168012

29. Mora A, García-Bernal D, Rodríguez-Lozano F.J, Sanz JL, Forner L, Ghilotti J, Lozano A, López-García, S. (2024). Biocompatibility, bioactivity and immunomodulatory properties of three calcium silicate-based sealers: an in vitro study on hPDLSCs. Clinical oral investigations, 28(8), 416. https://doi.org/10.1007/s00784-024-05812-1 PMid:38969964 PMCid:PMC11226489

30. ISO, ISO. 6876: 2012 Dentistry-root canal sealing materials. International Organization for Standardization: Geneva, Switzerland, 2012.

31. Torres FFE, Guerreiro-Tanomaru JM, Bosso-Martelo R, Espir CG, Camilleri J, Tanomaru-Filho M. Solubility, porosity, dimensional and volumetric change of endodontic sealers. Braz Dent J 2019;30:368-373. https://doi.org/10.1590/0103-6440201902607 PMid:31340227

32. Almeida MM, Rodrigues CT, Matos AA, Carvalho KK, Silva EJ, Duarte MA, et al. Analysis of the physicochemical properties, cytotoxicity and volumetric changes of AH Plus, MTA Fillapex and TotalFill BC Sealer. J Clin Exp Dent 2020;12:e1058-e1065. https://doi.org/10.4317/jced.57527 PMid:33262872 PMCid:PMC7680565

33. Ferreira CMA, de Lima CO, Pinto KP, Barbosa AFA, Fidalgo G, Sena G, Colaço MV, Barroso RC, Sassone LM, Silva EJNL. pH influences the volumetric change of calcium silicate- and epoxy resin-based root canal sealers: An ex vivo micro-CT study. Aust Endod J 2023;49(Suppl 1):194-201. https://doi.org/10.1111/aej.12716 PMid:36401559