Tous-Oviedo A, Navarro-Camejo A, Gonzalez-Arrieta D, Ramos-Manotas J, Barriga-Periñan L, Plazas-Román J. Calcium hydroxide removal using ultrasonic-activated acetic acid versus ultrasonic-activated sodium hypochlorite: A randomized ex vivo study with dual microscopy evaluation. J Clin Exp Dent. 2026;18(7):e854-64.

 

doi:10.4317/jced.63951

https://doi.org/10.4317/jced.63951

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References

1. Siqueira JF, Rôças IN. Present status and future directions: Microbiology of endodontic infections. Int Endod J. 2022;55(Suppl 3):512-30.
https://doi.org/10.1111/iej.13677
PMid:34958494

 

2. Mohammadi Z, Dummer PMH. Properties and applications of calcium hydroxide in endodontics and dental traumatology. Int Endod J. 2011;44:697-730.
https://doi.org/10.1111/j.1365-2591.2011.01886.x
PMid:21535021

 

3. Kim SK, Kim YO. Influence of calcium hydroxide intracanal medication on apical seal. Int Endod J. 2002;35:623-8.
https://doi.org/10.1046/j.1365-2591.2002.00539.x
PMid:12190902

 

4. Margelos J, Eliades G, Verdelis C, Palaghias G. Interaction of calcium hydroxide with zinc oxide-eugenol type sealers: a potential clinical problem. J Endod. 1997;23:43-8.
https://doi.org/10.1016/S0099-2399(97)80206-3
PMid:9594745

 

5. Vertucci FJ. Root canal morphology and its relationship to endodontic procedures. Endod Topics. 2005;10:3-29.
https://doi.org/10.1111/j.1601-1546.2005.00129.x

 

6. Peters OA, Laib A, Göhring TN, Barbakow F. Changes in root canal geometry after preparation assessed by high-resolution computed tomography. J Endod. 2001;27:1-6.
https://doi.org/10.1097/00004770-200101000-00001
PMid:11487156

 

7. Zehnder M. Root canal irrigants. J Endod. 2006;32:389-98.
https://doi.org/10.1016/j.joen.2005.09.014
PMid:16631834

 

8. Haapasalo M, Shen Y, Wang Z, Gao Y. Irrigation in endodontics. Br Dent J. 2014;216:299-303.
https://doi.org/10.1038/sj.bdj.2014.204
PMid:24651335

 

9. Madhubala MM, Srinivasan N, Ahamed S. Comparative evaluation of propolis and triantibiotic mixture as an intracanal medicament against Enterococcus faecalis. J Endod. 2011;37:1287-9.
https://doi.org/10.1016/j.joen.2011.05.028
PMid:21846550

 

10. Mancini M, Armellin E, Casaglia A, Cerroni L, Cianconi L. A comparative study of smear layer removal and erosion in apical intraradicular dentine with three irrigating solutions: a scanning electron microscopy evaluation. J Endod. 2009;35:900-3.
https://doi.org/10.1016/j.joen.2009.03.052
PMid:19482195

 

11. Rasimick BJ, Nekich M, Hladek MM, Musikant BL, Deutsch AS. Interaction between chlorhexidine digluconate and EDTA. J Endod. 2008;34:1521-3.
https://doi.org/10.1016/j.joen.2008.08.039
PMid:19026886

 

12. van der Sluis LWM, Versluis M, Wu MK, Wesselink PR. Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J. 2007;40:415-26.
https://doi.org/10.1111/j.1365-2591.2007.01243.x
PMid:17442017

 

13. Gu L, Kim JR, Ling J, Choi KK, Pashley DH, Tay FR. Review of contemporary irrigant agitation techniques and devices. J Endod. 2009;35:791-804.
https://doi.org/10.1016/j.joen.2009.03.010
PMid:19482174

 

14. Taşdemir T, Celik D, Er K, Yildirim T, Ceyhanli KT, Yeşilyurt C. Efficacy of several techniques for the removal of calcium hydroxide medicament from root canals. Int Endod J. 2011;44:505-9.
https://doi.org/10.1111/j.1365-2591.2011.01854.x
PMid:21276018

 

15. Bhuyan AC, Seal M, Pendharkar K. Effectiveness of four different techniques in removing intracanal medicament from the root canals: an in vitro study. Contemp Clin Dent. 2015;6:309-12.
https://doi.org/10.4103/0976-237X.161860
PMid:26321826 PMCid:PMC4549978

 

16. Ahmad M, Pitt Ford TR, Crum LA, Walton AJ. Ultrasonic debridement of root canals: acoustic cavitation and its relevance. J Endod. 1988;14:486-93.
https://doi.org/10.1016/S0099-2399(88)80105-5
PMid:3255774

 

17. Mozo S, Llena C, Forner L. Review of ultrasonic irrigation in endodontics: increasing action of irrigating solutions. Med Oral Patol Oral Cir Bucal. 2012;17:e512-6.
https://doi.org/10.4317/medoral.17621
PMid:22143738 PMCid:PMC3476090

 

18. Clarkson RM, Moule AJ, Podlich HM. The shelf-life of sodium hypochlorite irrigating solutions. Aust Dent J. 2001;46:269-76.
https://doi.org/10.1111/j.1834-7819.2001.tb00291.x
PMid:11838874

 

19. Barakat RM, Almohareb RA, Alsuwaidan M, Faqehi E, Alaidarous E, Algahtani FN. Effect of sodium hypochlorite temperature and concentration on the fracture resistance of root dentin. BMC Oral Health. 2024;24:233.
https://doi.org/10.1186/s12903-024-03954-y
PMid:38350980 PMCid:PMC10865544

 

20. Siqueira JF, Alves FRF, Versiani MA, Rôças IN, Almeida BM, Neves MAS, Sousa-Neto MD. Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by self-adjusting file, reciproc, and twisted file systems. J Endod. 2013;39:1044-50.
https://doi.org/10.1016/j.joen.2013.04.034
PMid:23880274

 

21. Wiseman A, Cox TC, Paranjpe A, Flake NM, Cohenca N, Johnson JD. Efficacy of sonic and ultrasonic activation for removal of calcium hydroxide from mesial canals of mandibular molars: a microtomographic study. J Endod. 2011;37:235-8.
https://doi.org/10.1016/j.joen.2010.11.019
PMid:21238809

 

22. Rödig T, Vogel S, Zapf A, Hülsmann M. Efficacy of different irrigants in the removal of calcium hydroxide from root canals. Int Endod J. 2010;43:519-27.
https://doi.org/10.1111/j.1365-2591.2010.01709.x
PMid:20536580

 

23. Macedo RG, Wesselink PR, Zaccheo F, Fanali D, Van Der Sluis LWM. Reaction rate of NaOCl in contact with bovine dentine: effect of activation, exposure time, concentration and pH. Int Endod J. 2010;43:1108-15.
https://doi.org/10.1111/j.1365-2591.2010.01785.x
PMid:20812947

 

24. Yücel AÇ, Gürel M, Güler E, Karabucak B. Comparison of final irrigation techniques in removal of calcium hydroxide. Aust Endod J. 2013;39:116-21.
https://doi.org/10.1111/j.1747-4477.2011.00326.x
PMid:24279657

 

25. Attur K, Joy MT, Karim R, Anil Kumar VJ, Deepika C, Ahmed H. Comparative analysis of endodontic smear layer removal efficacy of 17% ethylenediaminetetraacetic acid, 7% maleic acid, and 2% chlorhexidine using scanning electron microscope: an in vitro study. J Int Soc Prev Community Dent. 2016;6:S160-5.
https://doi.org/10.4103/2231-0762.189755
PMid:27652250 PMCid:PMC5022395

 

26. Pashley EL, Birdsong NL, Bowman K, Pashley DH. Cytotoxic effects of NaOCl on vital tissue. J Endod. 1985;11:525-8.
https://doi.org/10.1016/S0099-2399(85)80197-7
PMid:3867719